PXD066323

PXD066323 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Porcine Sertoli Cell-Derived Exosomes Drive Spermatogonial Stem Cell differentiation through PSMD11-p38 MAPK-Mediated Redox Signaling
Description	Spermatogonial stem cell fate decisions-self-renewal versus differentiation-are orchestrated by complex intercellular communication within the testicular niche. While paracrine signaling has been extensively characterized, the role of extracellular vesicles, especially in livestock, remains poorly understood. Here we demonstrate that primary porcine Sertoli cell-derived exosomes constitute a previously unrecognized regulatory mechanism controlling SSC differentiation through redox homeostasis. Using an ex vivo porcine testicular culture system that recapitulates the native SSC microenvironment, we show that porcine Sertoli cell exosomes promote SSC differentiation, evidenced by decreased expression of stemness markers (GFRA1, UCHL1) and increased differentiation markers (KIT, STRA8). Proteomic profiling revealed enrichment of antioxidant enzymes (SOD1, SOD2, CAT etc.,) within these exosomes. Mechanistically, PSMD11 was identified as a novel regulatory protein within the exosome to modulate SSC redox balance via the p38 MAPK pathway. The differentiation of porcine SSCs was impaired by PSMD11 knockdown, while was recovered by exogenous PSMD11, confirming its functional significance. These findings establish exosome-mediated redox regulation as a fundamental mechanism governing SSC fate decisions, with implications for understanding male fertility, developing therapeutic strategies for azoospermia, and improving reproductive efficiency in agricultural species.
HostingRepository	MassIVE
AnnounceDate	2025-07-19
AnnouncementXML	Submission_2025-07-19_03:54:41.703.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	songziqian
SpeciesList	scientific name: Sus scrofa; common name: pig; NCBI TaxID: 9823;
ModificationList	Carbamidomethyl; Deamidated; Oxidation
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-19 03:49:49	ID requested
⏵ 1	2025-07-19 03:54:42	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Cell fate determination, Exosomes, Redox signaling, Sertoli cells, Spermatogonial stem cells, DatasetType:Proteomics

submitter keyword: Cell fate determination, Exosomes, Redox signaling, Sertoli cells, Spermatogonial stem cells, DatasetType:Proteomics

Contact List

Kang Zou
contact affiliation	Nanjing Agricultural University
contact email	kangzou@njau.edu.cn
lab head
songziqian
contact affiliation	Nanjing Agricultural University
contact email	asong@msbiox.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v10/MSV000098563/