Klebsormidium nitens is an alga of the charophyte class considered to be a good model for studying the adaptation of plants to terrestrial life. Its genome has been completely sequenced and 16215 protein-coding genes have been predicted. This article presents the proteins identified by mass spectrometry in protein extracts from algae grown under standard conditions or after salt stress. A total of 1190 proteins were experimentally confirmed and 922 of them were classified according to their cellular location, molecular or biological function. Of these 922 proteins, 62 and 124 were found specifically in the control and salt-treated samples respectively. However, no specific function or location could not be assigned on the basis of the primary sequences. All the data are accessible and are of interest for phycologists as well as evolutionary plant biologists.