PXD066037

PXD066037 is an original dataset announced via ProteomeXchange.

Title	Azoospermia phenotype and scRNA-seq reveal hnRNPK as a factor essential for spermatogonial differentiation and meiotic entry in mice
Description	Continuous mitotic and meiotic cycles are essential prerequisites for germ cell development and male fertility. However, our understanding of the mechanisms underlying spermatogonial differentiation and meiosis in mammals remains limited. In this study, we employed a unique gene knockout mouse model to elucidate the critical role of heterogeneous nuclear ribonucleoprotein K (hnRNPK) in spermatogenesis and male fertility. Specifically, adult mice with germ cell-specific hnRNPK deletion exhibited complete infertility, with nearly a complete absence of spermatocytes in the seminiferous tubules, leaving only a small number of pre-leptotene spermatocytes. Further studies revealed that hnRNPK knockout disrupted spermatogonial differentiation and blocked entry into meiosis. Single-cell RNA sequencing analysis indicated that hnRNPK deletion led to cell cycle abnormalities in differentiating spermatogonia, which were unable to entry to meiosis and instead underwent apoptosis. Additionally, hnRNPK was found to primarily regulate post-transcriptional processes in germ cells. In the cytoplasm, hnRNPK interacts with ribosomal proteins (RPS3A, RPL13) and translation initiation factors (EIF4A1, EIF4A3) to regulate the initiation of translation. Following hnRNPK deletion, the translational efficiency of spermatogenesis-related genes was significantly reduced. In the nucleus, hnRNPK interacts with splicing factors and participates in the splicing of target genes related to differentiation and meiosis. This study highlights the functional role and mechanistic involvement of hnRNPK in spermatogenesis and provides valuable insights into the post-transcriptional regulatory mechanisms that govern this process.
HostingRepository	iProX
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-10_20:02:18.859.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huihui Gao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-10 20:02:02	ID requested
⏵ 1	2025-07-10 20:02:19	announced

Dataset with its publication pending

submitter keyword: hnRNPK, spermatogonia differentiation, meiosis entry, scRNA-seq, translation

Shuiqiao Yuan
contact affiliation	Huazhong University of Science and Technology
contact email	shuiqiaoyuan@hust.edu.cn
lab head
Huihui Gao
contact affiliation	Huazhong University of Science and Technology
contact email	berylgao2@163.com
dataset submitter

iProX dataset URI