PXD065986

PXD065986 is an original dataset announced via ProteomeXchange.

Title	Characterisation and screening analysis of haze-active proteins in thawed cloudy huyou (Citrus changshanensis) juice using a data-independent acquisition proteomic technique
Description	Turbidity loss is a major issue in thawed huyou (Citrus changshanensis) juice, and elucidating the precipitation composition is crucial for effective control. The present study employed data-independent acquisition proteomics to investigate the precipitation-related proteins in thawed cloudy huyou juice (TCHJ). A total of 1,268 precipitation-related proteins were identified, with the majority localised in the chloroplast and predominantly possessing P-loop NTPase domains. By cross-referencing various bioinformatics algorithms, 33 pivotal haze-active proteins were identified. The three-dimensional structures of haze-active proteins were constructed using artificial intelligence algorithms, and molecular docking simulations revealed that they interact with haze-active small molecules primarily through van der Waals forces and hydrogen bonds, leading to the formation of flocculation and precipitation in the TCHJ. According to fluorescence spectroscopy, the main static quench mode exists between the precipitated protein and the haze-activated small molecules; the binding ability and binding stability between the precipitated protein and the haze-activated small molecules were clarified; and the non-covalent binding mechanism between the two was verified. The findings of this study contribute to a deeper understanding of the precipitated protein system in TCHJ, providing technical support for future theoretical and process studies on the protein stability of TCHJ.
HostingRepository	iProX
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-09_23:03:33.013.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Boyuan Guan
SpeciesList	scientific name: Citrus suhuiensis; NCBI TaxID: 1008978;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-09 23:03:10	ID requested
⏵ 1	2025-07-09 23:03:33	announced

Dataset with its publication pending

submitter keyword: Cloudy huyou juice, precipitation, haze-active protein, DIA proteomics

Donghong Liu
contact affiliation	Zhejiang University
contact email	dhliu@zju.edu.cn
lab head
Boyuan Guan
contact affiliation	Zhejiang Univeristy
contact email	guanboyuanfs@163.com
dataset submitter

iProX dataset URI