PXD065882

PXD065882 is an original dataset announced via ProteomeXchange.

Title	C-Reactive Protein Is A Broad-Spectrum Capsule-Binding Receptor For Hepatic Capture of Blood-Borne Bacteria
Description	To understand how the liver executes the clearance of blood-borne bacteria, we sought to identify the receptor(s) recognizing the capsule of serotype-23F S. pneumoniae (Sp23F), one of the most predominant serotypes causing the childhood invasive infection. Our previous study has shown that Sp23F is rapidly captured by KCs into the liver of mice (Low virulence,LV). Our initial experiment showed that the hepatic immunity is blocked by free capsular polysaccharide of Sp23F (CPS23F). Consistently, the freshly isolated KCs from mouse liver showed significant adherence to LV Sp23F with normal mouse serum, but were poorly adherent to high virulence (HV) Sp8. We enriched CPS23F-binding protein(s) by affinity pulldown after incubation of CPS23F-coated beads with the membrane protein-enriched fraction of mouse liver nonparenchymal cells (NPCs) in the presence of 10% mouse serum. CPS of the HV serotype-8 S. pneumoniae (CPS8) was used as a negative control due to its poor binding to KCs
HostingRepository	iProX
AnnounceDate	2025-07-08
AnnouncementXML	Submission_2025-07-07_23:32:00.186.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Danyu Chen
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-07 23:31:39	ID requested
⏵ 1	2025-07-07 23:32:00	announced

Dataset with its publication pending

submitter keyword: Mouse, Plasma, capsule

Jingren Zhang
contact affiliation	Tsinghua University
contact email	zhanglab@tsinghua.edu.cn
lab head
Danyu Chen
contact affiliation	Tsinghua University
contact email	18275106941@163.com
dataset submitter

iProX dataset URI