PXD065783

PXD065783 is an original dataset announced via ProteomeXchange.

Title	Ubiquitin C-terminal Extension (CxUB) DUB cleavage assay
Description	Ubiquitin is a conserved modifier regulating the stability and function of numerous target proteins. In all eukaryotes, polyubiquitin precursors are generated and processed into ubiquitin monomers. The final ubiquitin unit always contains a C-terminal extension, but its physiological significance is unknown. Here we show that C-terminally extended ubiquitin, termed CxUb, is essential for stress resistance, mitophagy and longevity in yeast and worms. CxUb forms ubiquitin chains and binds to a novel region within the ubiquitin chain-elongating E4 enzyme Ufd2, which also functions during stress and aging. Ufd2 recognizes CxUb and conjugates it to substrate proteins, triggering their degradation. In contrast, CxUb is not required for basal housekeeping functions of the ubiquitin-proteasome system. These data suggest that the C-terminally extended ubiquitin encodes a functionally unique ubiquitin form, specialized for proteostasis defects, expanding the code of post-translational modification processes. For the intact protein detection, please find the corresponding target proteins: 1 NoDUb-T0 2 NoDUB-T10 3 NoDUB-T120 4 UBP3ia-T10 5 UBP3ia-T120 6 UBP3wt-T10 7 UBP3wt-T120 8 UBP14-T10 9 UBP14-T120 10 UBP15-T10 11 Ubp15-T120
HostingRepository	PRIDE
AnnounceDate	2025-09-24
AnnouncementXML	Submission_2025-09-24_02:22:00.102.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hendrik Nolte
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-07-04 04:13:16	ID requested
⏵ 1	2025-09-24 02:22:00	announced

Dataset with its publication pending

submitter keyword: DUBS, CxUB,Ubiquitination

Marcus Krüger
contact affiliation	CECAD Research Center/ Institute for Genetics University of Cologne Joseph-Stelzmann-Str. 26 50931 Cologne
contact email	marcus.krueger@uni-koeln.de
lab head
Hendrik Nolte
contact affiliation	Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany
contact email	h.nolte@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD065783

PRIDE project URI

• PRIDE
  1. PXD065783
     1. Label: PRIDE project
     2. Name: Ubiquitin C-terminal Extension (CxUB) DUB cleavage assay