PXD065570

PXD065570 is an original dataset announced via ProteomeXchange.

Title	Impact of Protein Modifications at Scissile Bonds on Trypsin Activity: Insights from the Major Peanut Allergen Ara h 1
Description	Trypsin is a key intestinal endopeptidase and the most widely used enzyme in proteomics workflows. However, the influence of protein modifications—commonly introduced during food processing—on intestinal digestibility and allergenic potential remains underexplored. Traditional methods for evaluating protease cleavage efficiency primarily consider the local amino acid sequence context, overlooking post-translational or process-induced modifications. In this study, a novel proteomic strategy was applied to assess trypsin cleavage efficiency at modified versus unmodified scissile sites within Ara h 1, a major peanut allergen. Among the 17 modified residues analyzed, 7 (41%) exhibited significant differences in cleavage efficiency (≥20%). Modifications such as methylation, methylation with carbamoylation, formylation, and dihydroxylation were associated with impaired digestion. Reduced cleavage at these sites may enhance the sensitizing potential of the resulting peptides. These findings support the development of machine learning algorithms and the mining of public proteomic datasets to systematically identify modification-driven digestion resistance or facilitation.
HostingRepository	PRIDE
AnnounceDate	2025-08-12
AnnouncementXML	Submission_2025-08-12_03:57:39.304.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD065570
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Teodora Cvetić
SpeciesList	scientific name: Arachis hypogaea (Peanut); NCBI TaxID: 3818;
ModificationList	L-methionine sulfoxide; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-27 12:53:56	ID requested
⏵ 1	2025-08-12 03:57:39	announced

10.6019/PXD065570;

10.1021/ACS.JAFC.5C05871;

submitter keyword: protein modifications

trypsin,allergic sensitization

Ara h 1

cleavage efficacy

gastrointestinal digestion

peanut allergen

Katarina Smiljanić
contact affiliation	University of Belgrade – Faculty of Chemistry, Centre of Excellence for Molecular Food Sciences & Department of Biochemistry
contact email	katicas71@gmail.com
lab head
Teodora Cvetić
contact affiliation	Faculty od Medicine, University of Belgrade
contact email	teodora.cvetic@med.bg.ac.rs
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065570
     1. Label: PRIDE project
     2. Name: Impact of Protein Modifications at Scissile Bonds on Trypsin Activity: Insights from the Major Peanut Allergen Ara h 1