This project aimed to assess the role of pharmacological inhibition of the aryl hydrocarbon receptor (AhR) on skeletal muscle and cortical bone health in aged mice. As part of a broader systems biology approach, proteomic profiling was employed to investigate molecular mediators of neuromuscular junction (NMJ) function in skeletal muscle tissue. The study compared vehicle-treated versus BAY2416964-treated aged mice, revealing key differentially expressed proteins involved in axonal development and NMJ stability.