We perform proteomic analysis on the parent strain SC1401 and the ΔqseB gene deletion mutant under standard growth conditions. The proteomic data will provide useful insights for the whole-system modeling of signal transduction and gene transcription regulation. This serves as an important foundation for further research into the role of QseB during Glaesserella parasuis infection, the pathogenic mechanisms of Glaesserella parasuis, as well as its prevention and control.