To detect the function of Kcr in MASLD development and prognosis, We analyzed the liver samples from normal and MASLD mice models. The inventory of crotonylated peptides detected in liver tissue was evaluated to gain further insights into their role in MASLD. We next quantified the changes of protein Kcr in response to HFD-fed MASLD mice relative to total protein abundance in normal liver tissues or VSG relative to total protein abundance in PAIR-FED liver tissues.