PXD065245

PXD065245 is an original dataset announced via ProteomeXchange.

Title	Inhibition of rhizobial cheaters by the host Medicago truncatula involves repression of symbiotic functions and induction of defense, dataset1.2
Description	In symbiotic plant-microbe interactions, the host invests considerable amounts of resources in the microbial partner. If the microbe does not reciprocate with a comparable symbiotic benefit it is regarded as a cheater. The host responds to cheaters with negative feedback mechanisms (sanctions) to prevent fitness deficits resulting from being exploited. We study sanctioning in the symbiosis between Medicago truncatula and the nitrogen-fixing rhizobium Sinorhizobium meliloti. We manipulate the exchange of resources between the partners in three ways, by (i) using mutant rhizobia defective in nitrogenase, (ii) replacing nitrogen in the atmosphere with argon gas; and (iii) supplying rich nitrogen fertilizer to the host. We follow the consequences of simulated cheating by examining the metabolome and proteome of both partners. We find that sanctioning occurs at different levels. In particular, we observe repression of essential symbiotic functions and changes in central metabolism that are likely to be relevant for microbial fitness and that could therefore contribute to sanctioning. In addition, sanctioning triggers a broad panel of defense markers. A thorough understanding of the multi-level phenomenon of sanctioning will be essential for its genetic dissection and for breeding of elite legume crops with efficient symbiosis.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-04_09:35:39.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Stumpe
SpeciesList	scientific name: Sinorhizobium meliloti 2011; NCBI TaxID: 1286640;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-19 23:40:04	ID requested
⏵ 1	2025-08-04 09:35:39	announced

Dataset with its publication pending

submitter keyword: Medicago truncatula

Sinorhizobium meliloti

nitrogen fixation

legume-rhizobium symbiosis

nodulation

sanctioning

nifH

Dieter Kressler
contact affiliation	Department of Biology University of Fribourg Switzerland
contact email	dieter.kressler@unifr.ch
lab head
Michael Stumpe
contact affiliation	Department of Biology, University of Fribourg
contact email	michael.stumpe@unifr.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD065245
     1. Label: PRIDE project
     2. Name: Inhibition of rhizobial cheaters by the host Medicago truncatula involves repression of symbiotic functions and induction of defense, dataset1.2