Reveal the multi-layered regulatory pattern of tobacco pattern triggered immunity, and systematically elucidate the pattern triggered immunity (PTI) mechanism of tobacco plants under the joint stress of microplastics and pathogens, especially in the multi-level regulatory network of signal transduction pathways, gene expression, and protein regulation. Through omics analysis, key regulatory factors in PTI response were identified, and a tobacco immune regulatory network model was constructed to reveal the dynamic regulatory mechanism of the plant immune system under different stress conditions.