We performed quisinostat drug bath on Toxoplasma gondii RH strain. Next, we carried out research using data-independent acquisition (DIA) quantitative proteomics technology. A total of 4,243 proteins were identified in the four samples, all of which were quantifiable. Proteins with significant differential expression were screened based on the criteria of fold change ≥1.5 (up-regulation >1.5-fold or down-regulation <0.67-fold) and P value <0.05. Among them, 282 proteins showed significant changes in expression levels, with 77 proteins significantly up-regulated and 205 proteins significantly down-regulated. Based on the above data, systematic bioinformatics analysis was conducted for all identified proteins.