PXD064892

PXD064892 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic and immunoinformatic analysis reveals novel allergen candidates in blood clams
Description	Shellfish allergy is a significant public health concern, yet the molecular basis of allergenicity in molluscs remains poorly defined. In this study, we performed an in-depth proteomic and immunoinformatic analysis of two widely consumed blood clam species, Anadara broughtonii and Tegillarca granosa, to identify and characterize novel allergen candidates. Using high-resolution tandem mass spectrometry, we generated comprehensive protein profiles and applied three complementary allergen prediction algorithms (SEP-AlgPro™, AllerCatPro,and AlgPred 2.0) to screen for potential IgE-binding proteins. Immunoblotting with sera from shrimp-sensitized individuals confirmed IgE reactivity for nine proteins, including catalase and several hemoglobin isoforms—none of which are currently recognized as molluscan allergens. Homology modeling and sequence alignment revealed conserved structural motifsimplicated in allergenicity and suggested cross-reactivity with known crustacean allergens. These findings expand the repertoire of putative molluscan allergens and demonstrate theutility of integrated proteomic and immunoinformatic strategies for allergen discovery.
HostingRepository	PRIDE
AnnounceDate	2025-08-11
AnnouncementXML	Submission_2025-08-11_14:33:23.283.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD064892
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Teodora Cvetić
SpeciesList	scientific name: Mollusca; NCBI TaxID: 6447;
ModificationList	carbamoylated residue; deamidated residue
Instrument	Orbitrap Exploris 240

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 12:27:03	ID requested
⏵ 1	2025-08-11 14:33:23	announced

Publication List

10.6019/PXD064892;
10.1021/ACS.JAFC.5C05871;

10.6019/PXD064892;

10.1021/ACS.JAFC.5C05871;

Keyword List

submitter keyword: allergen prediction
blood clam
hemoglobin
immunoproteomics
IgE-binding
shellfish allergy
shrimp allergen cross-reactivity

submitter keyword: allergen prediction

blood clam

hemoglobin

immunoproteomics

IgE-binding

shellfish allergy

shrimp allergen cross-reactivity

Contact List

Tanja Ćirković Veličković
contact affiliation	University of Belgrade – Faculty of Chemistry, Centre of Excellence for Molecular Food Sciences & Department of Biochemistry, Belgrade, Serbia Serbian Academy of Sciences and Arts, Belgrade, Serbia
contact email	tanja.cirkovic16@gmail.com
lab head
Teodora Cvetić
contact affiliation	Faculty od Medicine, University of Belgrade
contact email	teodora.cvetic@med.bg.ac.rs
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD064892

PRIDE project URI

Repository Record List

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