PXD064874

PXD064874 is an original dataset announced via ProteomeXchange.

Title	Neurons Release Serine to Support mRNA Translation in Pancreatic Cancer
Description	Pancreatic ductal adenocarcinoma (PDAC) tumors have a nutrient-poor, desmoplastic, and highly innervated tumor microenvironment. Although neurons can release stimulatory factors to accelerate PDAC tumorigenesis, the metabolic contribution of peripheral axons has not been explored. We found that peripheral axons release serine (Ser) to support the growth of exogenous Ser (exSer)-dependent PDAC cells during Ser/Gly (glycine) deprivation. Ser deprivation resulted in ribosomal stalling on two of the six Ser codons, TCC and TCT, and allowed the selective translation and secretion of nerve growth factor (NGF) by PDAC cells to promote tumor innervation. Consistent with this, exSer-dependent PDAC tumors grew slower and displayed enhanced innervation in mice on a Ser/Gly-free diet. Blockade of compensatory neuronal innervation using LOXO-101, a Trk-NGF inhibitor, further decreased PDAC tumor growth. Our data indicate that axonal-cancer metabolic crosstalk is a critical adaptation to support PDAC growth in nutrient poor environments
HostingRepository	PRIDE
AnnounceDate	2025-07-07
AnnouncementXML	Submission_2025-07-07_06:53:07.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miljan Kuljanin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-06-11 05:52:51	ID requested
⏵ 1	2025-07-07 06:53:08	announced

10.1016/J.CELL.2020.10.016;

10.1016/j.cell.2020.10.016;

Banh RS, Biancur DE, Yamamoto K, Sohn ASW, Walters B, Kuljanin M, Gikandi A, Wang H, Mancias JD, Schneider RJ, Pacold ME, Kimmelman AC, Neurons Release Serine to Support mRNA Translation in Pancreatic Cancer. Cell, 183(5):1202-1218.e25(2020) [pubmed]

submitter keyword: Neurons, Proteomimcs, Pancreatic Cancer, mRNA, Serine

Joseph Mancias
contact affiliation	Division of Genomic Stability and DNA Repair, Department of Radiation Oncology, Dana-Farber Cancer Institute, Boston, MA 02215, USA, Hale Family Pancreatic Cancer Research Center, Dana-Farber Cancer Institute, Boston, MA 02215, USA
contact email	Joseph_Mancias@DFCI.HARVARD.EDU
lab head
Miljan Kuljanin
contact affiliation	Flare Therapeutics
contact email	mkuljanin@flaretx.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD064874

PRIDE project URI

• PRIDE
  1. PXD064874
     1. Label: PRIDE project
     2. Name: Neurons Release Serine to Support mRNA Translation in Pancreatic Cancer