Given the pivotal role of Tat in HIV-1 latency, we performed RNA pull-down assays in latently HIV-1-infected J-Lat 10.6 cells using biotinylated RNA probes (including a control RNA probe and a Tat-specific RNA probe) . Cell lysates were incubated with streptavidin agarose resin pre-bound to Tat RNA probes, and the enriched Tat RNA-binding proteins were separated by SDS-PAGE and analyzed by liquid chromatography mass spectrometry (LC-MS/MS)