HCT116 Cells were CRISPR-Edited, selected and sub-cloned for homocygous endogenous tagged clones. SPATA5 (Q8NB90), SPATA5L1 (Q9BVQ7), CINP (Q9BW66) and C1ORF109 (Q9NX04) were tagged with FKBP12F36V and subjected to 50nM of dTAGV-1 PROTAC molecule or equimolar DMSO solvent control for the indicated times.