T. fuciformis is an edible fungus prized for its culinary value. The polysaccharide content of T. fuciformis grown on a Cyclobalanopsis substrate (TY3) was significantly higher than those grown on a mixed substrate (TF1) made of wheat bran and cottonseed hull. proteomics were used to assess the effects of the lignocellulose (consists of cellulose, hemicellulose and lignin) in different growth substrates on the polysaccharide content of Tremella fuciformis and its formation mechanism.