To investigate the interactome of the human translocase of the outer mitochondrial membrane (TOM) complex, we performed immunoaffinity purification of TOMM22-FLAG from HEK293T cells followed by LC-MS/MS analysis. This approach allowed us to identify proteins that co-purify with the TOM complex under native conditions. Our primary aim was to uncover human-specific interactors not previously described in yeast, with a particular focus on factors potentially involved in mitochondrial protein import regulation and quality control. The dataset provides a resource for understanding conserved and divergent aspects of TOM complex composition and function in human cells.