The Schlafen (SLFN) family of nucleic acid binding proteins play an important role in gene regulation. In response to environmental stress, many SLFN members are activated for RNA cleavage and translational regulation. To characterize the RNA cleavage activity of this protein family, we produced recombinant human SLFN11 and SLFN14 protein. We utilized mass spectrometry for protein identification to confirm the identity of our recombinant SLFN protein samples.