PXD063957

PXD063957 is an original dataset announced via ProteomeXchange.

Title	Functional characterisation of Cullin-Ring-Ligases in Plasmodium falciparum
Description	Ubiquitination is the key post-translational modification in eukaryotic cells that governs protein degradation, localisation, and activity. This process results in the tagging of ubiquitin to protein substrates by a concerted enzyme cascade of E1, E2 and E3 enzymes. The largest superfamily of these proteins includes the Cullin-Ring-Ligase (CRL) complexes of E3 ligases. Plasmodium falciparum, the causative agent of the most severe form of malaria in humans, encodes the critical proteins required for ubiquitination, but we do not yet understand the function of this pathway. Here the P. falciparum CRL E3 ligases were characterised to reveal an essential but minimal repertoire controlled by two Cullin scaffolds. A PfCullin1-linked CRL complex was identified as being required for parasite inner-membrane biogenesis and DNA replication. This complex recruits only one substrate receptor, compared to the ~70 receptors used interchangeably by human cells. A second CRL complex functioning through a PfCullin4 scaffold was identified that utilised a previously unidentified adaptor protein and receptors to support DNA replication. These results show that the P. falciparum E3 ubiquitin ligases play an essential role in the biology of this important infectious agent.
HostingRepository	PRIDE
AnnounceDate	2025-07-11
AnnouncementXML	Submission_2025-07-11_09:38:07.842.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: 36329;
ModificationList	ubiquitination signature dipeptidyl lysine
Instrument	Orbitrap Eclipse; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-15 01:17:58	ID requested
⏵ 1	2025-07-11 09:38:08	announced

10.1038/S41598-025-05342-0;

submitter keyword: Plasmodium falciparum, E3-ligases, Malaria, Ubiquitin

Prof. Alan Cowman
contact affiliation	The Walter and Eliza Hall institute of Medical Research Professor Department of Medical Biology Faculty of Medicine, Dentistry and Health Sciences The University of Melbourne (lab head)
contact email	cowman@wehi.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063957

PRIDE project URI

• PRIDE
  1. PXD063957
     1. Label: PRIDE project
     2. Name: Functional characterisation of Cullin-Ring-Ligases in Plasmodium falciparum