Seven Rambouillet-crossbred wethers were utilized to examine the impact of copper (Cu) supplementation on plasma, bile, liver, duodenum, jejunum, and ileum tissue Cu concentrations and Cu trafficking proteins abundance in the duodenum and liver. Wethers were blocked by body weight and assigned to one of two treatments: 1) control: no supplemental Cu and 2) +Cu: 12 mg of supplemental Cu/kg DM. After receiving treatments for 29 d, all wethers were transported to a USDA-inspected abattoir and harvested. Following harvest, blood, bile, liver, duodenum, jejunum, and ileum samples were collected for Cu concentration analysis. Additional duodenum and liver samples were collected for proteomic analysis.