This study aims to investigate the protein expression profiles in a murine model of dextran sulfate sodium (DSS)-induced colitis using advanced Astral-DIA quantitative proteomics technology. A total of 12 colon tissue samples were analyzed, including 6 from healthy control mice and 6 from DSS-treated mice with induced colitis. ​​Experimental Design​​ ​​Species​​: Mus musculus (C57BL/6 strain). ​​Tissue Source​​: Colon tissues were dissected, snap-frozen in liquid nitrogen, and homogenized to extract proteins. ​​Groups​​: ​​Control Group​​: Healthy mice without intervention. ​​DSS Group​​: Mice subjected to 2.5% DSS administration for 7 days to induce colitis, validated by histopathological assessment.