PXD063603

PXD063603 is an original dataset announced via ProteomeXchange.

Title	β-catenin functions as a molecular adapter for disordered cBAF interactions
Description	BAF (SWI/SNF) chromatin remodelers engage binding partners to generate sitespecific DNA accessibility. However, the basis for interactions between BAF and divergent binding partners has remained unclear. Here we tested the hypothesis that scaffold proteins augment BAF’s binding repertoire by examining β-catenin and steroidogenic factor-1 (SF-1, NR5A1), a β-catenin–dependent transcription factor central to steroid production. In steroidogenic cell lines, BAF inhibition rapidly opposed overlapping SF-1/β-catenin enhancer occupancy, thereby impairing SF-1 target activation, steroid production, and SF-1/β-catenin autoregulation. These effects arise due to β-catenin’s role as an adapter between SF-1 and an intrinsically disordered region (IDR) of the canonical BAF (cBAF) subunit ARID1A. In contrast to exclusively IDR-driven mechanisms, folded Armadillo repeats on β-catenin were required to bind cBAF. β-catenin similarly linked cBAF to SOX2, FOXO3, YAP1, and CBP/p300. Visualization of contacts highlighted β-catenin’s central role in IDR-dependent interaction of cBAF with diverse transcription factors and chromatin regulators. The cross-linking dataset was used to validate the interaction interface obtained with computational modelling.
HostingRepository	PRIDE
AnnounceDate	2025-07-11
AnnouncementXML	Submission_2025-07-11_00:50:19.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Růžena Filandrová
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-05 06:16:47	ID requested
⏵ 1	2025-07-11 00:50:19	announced

10.1016/J.MOLCEL.2025.06.026;

submitter keyword: transcription regulators,Unstructured protein, chromatin remodeling, steroid hormones, IDRs

Václav Veverka
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague
contact email	vaclav.veverka@uochb.cas.cz
lab head
Růžena Filandrová
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, Prague
contact email	ruzena.filandrova@uochb.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063603

PRIDE project URI

• PRIDE
  1. PXD063603
     1. Label: PRIDE project
     2. Name: β-catenin functions as a molecular adapter for disordered cBAF interactions