PXD063543

PXD063543 is an original dataset announced via ProteomeXchange.

Title	Molecular Landscape of Sex and Modality-Specific Exercise Adaptation in Human Skeletal Muscle through Large-Scale Multi-OMICs Integration
Description	We investigated the molecular mechanisms of exercise adaptations in human muscle by integrating genome, methylome, transcriptome, and proteome data from over 1,000 participants (2,340 muscle samples). We identified distinctive signatures associated with maximal oxygen consumption (VO 2max ), and multi-omics integration uncovered five key genes as robust exercise markers across layers, with transcription factors functioning as activators, synergizing with DNA methylation to regulate gene expression. Minimal sex-differences were observed, while modality-specific analysis highlighted distinct pathways for aerobic and resistance exercise, contrasting with muscle disuse patterns. Finally, we have created a webtool namely OMAx (https://sarah-voisin.shinyapps.io/OMAEx/) featuring our individual omics and integration analysis. These findings provide a comprehensive multi-omic framework for understanding exercise-induced molecular adaptations, offering insights into muscle health, cardiorespiratory fitness, and their roles in aging and disease prevention.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_18:56:10.916.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Han Lee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-05-01 18:53:40	ID requested
⏵ 1	2025-05-06 18:56:11	announced

10.1101/2024.07.14.603458;

submitter keyword: transcriptome, proteomics, methylome, exercise, cardiorespiratory fitness,multi-omics, skeletal muscle

Macsue Jacques
contact affiliation	Monash University
contact email	Macsue.jacques@monash.edu
lab head
Han Lee
contact affiliation	Monash Proteomics & Metabolomics Facility
contact email	han.lee@monash.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD063543

PRIDE project URI

• PRIDE
  1. PXD063543
     1. Label: PRIDE project
     2. Name: Molecular Landscape of Sex and Modality-Specific Exercise Adaptation in Human Skeletal Muscle through Large-Scale Multi-OMICs Integration