PXD063435

PXD063435 is an original dataset announced via ProteomeXchange.

Title	LC-MSMS of Brucella infected RAW264.7 cells
Description	The RAW264.7 cells (2x105 cells/ml) were seeded in 100 mm cell culture dishes in DMEM containing 10% FBS. When the cell density reached 70%, cells were infected with B. abortus A19 (MOI=200) for 4 h, and the cell medium then was changed to DMEM containing 10% FBS with gentamicin (50 ng/μl) for 1 h. The medium subsequently was changed to DMEM containing 10% FBS with gentamicin (25 ng/μl) for 24 h at which time cells were harvested and placed at -80°C. Subsequently, the cells lysates were analyzed by protein liquid chromatography-mass spectrometry (LC-MS/MS)
HostingRepository	PRIDE
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-10_02:52:09.473.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanhao Yang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-29 02:53:13	ID requested
⏵ 1	2025-07-10 02:52:09	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, RAW264.7 cell, lactate,Brucella abortus

Yuanhao Yang
contact affiliation	College of Veterinary Medicine, Northwest A&F University
contact email	yangyuanhao3735@163.com
lab head
Yuanhao Yang
contact affiliation	Northwest A&F University
contact email	yangyuanhao3735@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD063435

PRIDE project URI

• PRIDE
  1. PXD063435
     1. Label: PRIDE project
     2. Name: LC-MSMS of Brucella infected RAW264.7 cells