Mitochondrial biogenesis and inheritance must be carefully regulated alongside cell division to ensure proper mitochondrial function and cell survival. The dynamics of the mitochondrial network, including fusion and fission, play a crucial role in mitochondrial inheritance by facilitating the distribution and quality control of mitochondria. Here, we investigate the consequences of impaired mitochondrial fusion through depletion of Fzo1, which is essential for mitochondrial outer membrane fusion in s. cerevisiae. We use the AID (auxin-induced degron) system, which enables induced protein degradation and monitor changes in the proteome up to 21 hours after depletion.