Centrosome-enriched fractions of Jurkat E6-1 human T cells (JK), human embryonic kidney (HEK) 293 cells, resting human T lymphoblasts (LT CTRL) and activated human T lymphoblasts (LT ACT), with one biological replicate for each condition, were analysed by label-free LC-MS/MS. For further details, refer to reference (Lozano-Prieto M, Camafeita E, Jorge I, Laguillo-Gómez A, Barrero-Rodríguez R, Devesa CA, Pertusa C, Calvo E, Sánchez-Madrid F, Vázquez J, Martin-Cofreces NB. In-gel protein digestion using acidic methanol produces a highly selective methylation of glutamic acid residues. J Proteomics. 2024. 304:105229).