O-linked β-N-acetylglucosamine (O-GlcNAc) modification, a posttranslational modification (PTM) enriched in the nucleus and cytoplasm, is acknowledged to substantially contribute to the regulation of chromatin assembly and gene expression. studies have indicated that O-GlcNAc modification plays an important role in the process of cellular senescence. To integratively illustrate the regulatory role of O-GlcNAc modification in the oncogene-induced senescence (OIS) process, we employed a chemical reporter-based multi-omics strategy for a time-series proteomic profiling and genome-wide mapping of O-GlcNAc chromatin-associated proteins (OCPs) in human primary fibroblasts