To investigate the underlying mechanisms of Scutellariae Radix and Atractylodis Macrocephalae Rhizoma (SA) in treating preeclampsia (PE), particularly its pathogenic regulation and pharmacodynamic actions, we performed a comprehensive proteomic analysis using placental tissues from three experimental groups of pregnant rats: control, PE model, and medium-dose SA treatment. The extracted proteomes were analyzed using stable isotope dimethyl labeling coupled with HPLC-MS/MS technology. This integrated approach enabled systematic identification and quantification of differentially expressed proteins associated with PE pathogenesis and SA-mediated therapeutic effects, providing molecular-level insights into the biological responses to SA intervention.