To identify proteins (especially E3 ligases) potentially binding to human LC3 protein, Caki-1 cells were used to perform LC-MS/MS analysis based on anti-LC3 (Proteintech, 14600-1-AP, 1:50) IP products (gel bands cutted after SDS-PAGE and CBB staining).Anti-IgG IP products were used as a negative control. Three independent experiments were performed.