PXD062971

PXD062971 is an original dataset announced via ProteomeXchange.

Title	Identification and validation of a tear fluid-derived protein biomarker signature in patients with Amyotrophic Lateral Sclerosis
Description	The diagnosis of Amyotrophic Lateral Sclerosis (ALS) primarily relies on clinical findings and remains challenging, particularly in the early stages of the disease, where characteristic symptoms may be subtle and nonspecific. Therefore, the development of disease-specific and clinically validated biomarkers is crucial to enhance diagnostic precision and optimize patient management. Here, we explored tear fluid (TF) as a promising biomarker source for ALS, given its accessibility, prior evidence of discriminative power in other neurodegenerative diseases, and its anatomical proximity to the brainstem as an important site of neurodegeneration. Using a discovery-based approach, we profiled protein abundance in TF of 49 ALS patients and 54 controls via data-independent acquisition mass spectrometry (DIA-MS) Biostatistical analysis and machine learning identified a signature of six proteins with diagnostic potential (AUROC = 66.2%).
HostingRepository	PRIDE
AnnounceDate	2025-09-08
AnnouncementXML	Submission_2025-09-07_19:33:20.805.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-04-15 03:13:26	ID requested
⏵ 1	2025-09-07 19:33:29	announced

10.1186/s40478-025-02109-6;

Scholl LS, Demleitner AF, Riedel J, Adachi S, Neuenroth L, Meijs C, Tzeplaeff L, Caldi Gomes L, Galhoz A, Cordts I, Lenz C, Menden M, Lingor P, Identification and validation of a tear fluid-derived protein biomarker signature in patients with amyotrophic lateral sclerosis. Acta Neuropathol Commun, 13(1):187(2025) [pubmed]

submitter keyword: proteomics, diagnosis,Amyotrophic lateral sclerosis, tear fluid, biomarker

Christof Lenz
contact affiliation	Core Facility Proteomics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD062971

PRIDE project URI

• PRIDE
  1. PXD062971
     1. Label: PRIDE project
     2. Name: Identification and validation of a tear fluid-derived protein biomarker signature in patients with Amyotrophic Lateral Sclerosis