This dataset assesses the effects of S-DELE1 association on the auto-phosphorylation of human, heme-bound HRI. Human HRI and DELE1 were expressed in EIF2alpha-phospho-resistant cells supplemented with hemin. HRI was affinity purified, the elution subjected to denaturing gel electrophoresis, and HRI phosphorylation was assessed by MS. The data set identifies S-DELE1 induced phosphorylation sites in the N-terminal domain of HRI, its two kinase domains, and the kinase insert.