This project is to identify the binding partners of the OMM anchored ubiquitin-fusion degradation substrate (mitoUFD) in comparison with the mitoGFP control that lacks the ubiquitin fusion as the degradation signal. In addition, we performed RNAi knockdown for bcat-1, lars-1 and gcn-2 compared to the L4440 RNAi control to identify the changes of mitoUFD binding partners upon disruption of leucine catabolism or sensing.