TRIM24 (Tripartite Motif-Containing 24) is a key transcriptional co-regulator involved in various cellular processes, including chromatin remodeling and gene expression. However, its role in cardiomyocytes remains largely unexplored. In this study, we investigated the global proteomic changes associated with TRIM24 downregulation in neonatal rat ventricular cardiomyocytes (NRVCMs) to better understand its function in cardiac biology. NRVCMs were transduced by an Adenovirus with a synthetic microRNA targeting TRIM24. Protein isolated from the NRVCMs transduced with a scrambled microRNA was used as control.