In this study we conducted proteomics analyses of historical and contemporary Bombyx mori cocoon shells to case-study the human-driven introduction and diversification of this species in Europe. We employed LC-MS/MS analyses protocols for 81 cocoon shell samples to identify that the cocoon shell of this species contains on average 98±13 (Mean±SD) proteins, Our proteomic datasets provide a valuable foundation for advancing further exploitations of silkworm cocoon shells in multiple scientific perspectives.