Using HCT116 wild-type cells as a blank control, we divided 3×Flag-IP6K2 knockin HCT116 cells into two groups: a negative control (untreated) and an experimental group (treated with 3% DSS for 4 hours). After treatment, cells were lysed, and Flag-tagged IP6K2 and its interacting proteins were immunoprecipitated (IP) using anti-Flag beads. Subsequently, bound proteins were competitively eluted with a FLAG peptide. The eluates were then analyzed by mass spectrometry (MS) to identify DSS-induced alterations in the IP6K2 interactome.