5×106 PaTu 8988t cells were spread into 10cm dish and then replaced into 5ml serum-free medium after being attached to the wall. At the same time, 5ml of the supernatant of PCAF-Ctrl, PCAF-CPXM2-WT and PCAF-CPXM2-mut stable cell strains were added to each group. After 8 hours, proteins from each group were collected for phosphorylated protein enrichment. The phosphorylated protein enrichment kit was used for this process, followed by protein mass spectrometry analysis of the signaling pathway of pancreatic cancer cells activated by collagen topology mediated by CPXM2.