PXD062246

PXD062246 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Interaction factors of DDB2 immunopurified from UV irradiated cells
Description	HEK293F cells expressing FLAG-tagged DDB2 were exposed with UV-C. After 15 min incubation, cell lysate was prepared and chromatin fraction was fractionated. The resulting chromatin sample was digested by micrococcal nuclease treatment. The cellular UV-DDB (DDB1-DDB2 complex) bound to chromatin was then immunopurified by agarose beads conjugated with the anti-FLAG M2 antibody. After DDB2 immunopurification, elute fraction was subjected to SDS-PAGE and stained by CBB. Target proteins in the excised gels were in-gel digested with trypsin and identified by nano LC-MS/MS analysis.
HostingRepository	jPOST
AnnounceDate	2025-11-11
AnnouncementXML	Submission_2025-11-11_03:14:44.272.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lumi Negishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; alpha-amino acetylated residue; unknown modification
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-25 23:26:52	ID requested
⏵ 1	2025-11-11 03:14:44	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: SDS-PAGE, Pulldown assay, UV-damaded DNA binding proteins,

submitter keyword: SDS-PAGE, Pulldown assay, UV-damaded DNA binding proteins,

Contact List

Hitoshi Kurumizaka
lab head
Lumi Negishi
contact affiliation	The University of Tokyo
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003722/