PXD062206

PXD062206 is an original dataset announced via ProteomeXchange.

Title	Extensive PFAS accumulation by human gut bacteria
Description	Per- and polyfluoroalkyl Substances (PFAS) – the so-called ‘forever chemicals’ – are a major cause of environmental and health concern due to their toxicity and long-term persistence1,2. Yet, no efficient mechanisms for their removal have been identified. Here we report bioaccumulation of PFAS by several gut bacterial species over a wide range of concentrations from nanomolar up to 500 μM. For bioaccumulating Bacteroides uniformis, a highly prevalent species, we estimate intracellular PFAS concentration in the mM range – above that of most native metabolites. Despite this high bioaccumulation, B. uniformis cells could grow appreciably up to 250 μM perfluorononanoic acid (PFNA) exposure. Escherichia coli, which accumulated PFAS to a much lesser extent, substantially increased PFAS bioaccumulation when lacking TolC efflux pump indicating trans-membrane transport in PFAS bioaccumulation. Electron microscopy and cryogenic Focused Ion Beam-Secondary Ion Massspectrometry revealed distinct morphological changes and intracellular localisation of PFNA aggregates. Bioaccumulation of PFAS and transmembrane transport is also evident in proteomics, metabolomics, thermal proteome profiling, and mutations following adaptive laboratory evolution. In an in vivo context, mice colonized with human gut bacteria showed, compared to germ-free controls or those colonized with low-bioaccumulating bacteria, higher PFNA levels in excreted feces. As the gut microbiota is a critical interface between exposure and human body, our results have implications for understanding and utilizing microbial contribution to PFAS clearance.
HostingRepository	PRIDE
AnnounceDate	2025-04-28
AnnouncementXML	Submission_2025-04-28_03:10:49.781.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandip Kumar Patel
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Bacteroides uniformis; NCBI TaxID: 820;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-25 05:53:00	ID requested
⏵ 1	2025-04-28 03:10:50	announced

10.1101/2024.09.17.613493;

submitter keyword: gut bacteria,PFAS

Kiran Patil
contact affiliation	MRC Toxicology Unit, University of Cambridge
contact email	kp533@mrc-tox.cam.ac.uk
lab head
Sandip Kumar Patel
contact affiliation	Proteomics Core Facility, MRC Toxicology Unit, University of Cambridge
contact email	proteomics@mrc-tox.cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062206
     1. Label: PRIDE project
     2. Name: Extensive PFAS accumulation by human gut bacteria