Plasma membrane-localized pattern recognition receptors (PRRs) percieve host-derived phytocytokines (e.g. systemin) and microbe-associated molecular patterns (MAMPs; e.g. flg22 and chitin). Upon ligand binding, activation of PRRs induces similar early phosphorylation-regulated responses including extracellular alkalization and reactive oxygen species burst. However, despite similar early signaling events, the downstream responses are distinct: MAMPs trigger plant innate immunity, and the wound response is elicited by systemin. Therefore, the question arises as to how systemin-mediated responses differ from that of MAMPs, and how specificity is achieved. Here we used a Solanum peruvianum suspension cell culture to analyze phospho-proteomic responses triggered by 20nM flg22 and 15 ug/mL chitin compared to mock(water) treatment. Samples were analyzed in a time series of 0,1,2,5,15 and 45 min after elicitor treatment in six biological replicates. Sample numbering scheme:TreatmentTimeReplicate(e.g., F153 corresponds to flg22 treatment, 15min, the 3rd biological replicate; "C" and "H" are for chitin and water-treated samples,respectively.)