PXD062043

PXD062043 is an original dataset announced via ProteomeXchange.

Title	Proteomic Remodeling During Tumor Cell-Induced Platelet Aggregation Unveils Metastatic Drivers in Colorectal Cancer
Description	Colorectal cancer (CRC) is frequently associated with metastasis, resulting in high mortality rates. Platelets are known to play a crucial role in the metastatic cascade influencing tumor microenvironment remodeling, promoting cell transformation, facilitating metastatic niche formation, and shielding circulating tumor cells from immune surveillance. However, platelet proteomic alterations during tumor cell-induced platelet aggregation (TCIPA) remain largely unexplored. This study aims to characterize the proteomic profile of TCIPA in CRC using an in vitro model that recapitulates key aspects of CRC metastasis. TCIPA was assessed via light transmission aggregometry using an in vitro model incorporating paired primary and metastatic cell cultures. Stable Isotope Labeling with Amino Acids in Cell culture (SILAC) allowed the discrimination of healthy platelet and tumor cell proteomes prior to and following TCIPA. Data-independent acquisition mass spectrometry was employed to analyze intra- and extracellular tumor and platelet proteomes. Comparative proteomic profiling was performed using a range of bioinformatic analyses, including clustering, differential expression, and Gene Set Enrichment Analyses (GSEA). Comparison of the baseline proteome profiles of SW480 and SW620 cell lines identified 263 significant differentially expressed proteins. The GSEA demonstrated enrichment of the ‘epithelial-mesenchymal transition’ gene set in SW480 cells. While SW480 exhibited rapid TCIPA, SW620 did not consistently interact with healthy platelets. Following TCIPA, 34 tumor proteins showed differential expression compared to their naïve status. Notably, 17 of these proteins were significantly associated with CRC progression, particularly in the promotion of EMT, metastasis, tumor cell survival, proliferation, and metabolic reprogramming. This study successfully characterized the proteomic profiles of platelets, platelet secretomes, and colorectal tumor cells following TCIPA-induced activation. The findings highlight the significant role of several tumor proteins and their metabolic effects in colorectal cancer progression.
HostingRepository	PRIDE
AnnounceDate	2026-04-02
AnnouncementXML	Submission_2026-04-02_00:56:39.183.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thorben Sauer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-19 12:00:38	ID requested
⏵ 1	2026-04-02 00:56:41	announced

10.1186/s12935-026-04282-7;

Sauer T, Gruner C, Kern K, Rackisch A, Tischner L, Schulz K, Ostermann J, Cohrs L, Kohl M, Verschoor A, Gemoll T, Proteomic remodeling during tumor cell-induced platelet aggregation unveils metastatic drivers in colorectal cancer. Cancer Cell Int, 26(1):(2026) [pubmed]

submitter keyword: TCIPA, proteomics, EMT, DIA-MS,Platelets, metastasis, colorectal cancer

Prof. Dr. Timo Gemoll
contact affiliation	University of Lübeck Section for Translational Surgical Oncology & Biobanking Department of Surgery Ratzeburger Allee 160, BMF 23558 Lübeck, Germany
contact email	timo.gemoll@uni-luebeck.de
lab head
Thorben Sauer
contact affiliation	Department of Surgery, Laboratory for Surgical Research, University Hospital Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Lübeck Germany
contact email	thorben.sauer@student.uni-luebeck.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD062043
     1. Label: PRIDE project
     2. Name: Proteomic Remodeling During Tumor Cell-Induced Platelet Aggregation Unveils Metastatic Drivers in Colorectal Cancer