Sepsis-induced myocardial dysfunction is associated with high mortality in sepsis. However, the underlying mechanisms of sepsis-induced myocardial dysfunction remain unclear. The heart is an energy-demanding organ. Under basal physiological conditions, cardiomyocytes rely primarily on energy provided by fatty acid oxidation. In this study, we identified a total of 3,579 proteins and 8,519 Kcr sites, averaging 2,794 proteins and 5,550 Kcr sites per sample. We established a deep learning model. The highest-ranked functional Kcr site was CPT1B K321cr. Multiple sequence alignment of CPT1B from Rattus norvegicus, Homo sapiens, and Mus musculus revealed high similarity among these protein sequences. Additionally, the functional prediction of pFKcr was validated by the evolutionary conservation of the rat CPT1B K321 site in mice and humans.