PXD061849-1

PXD061849 is an original dataset announced via ProteomeXchange.

Title	Selective Inhibition of MTHFD1/2 in T Cells from Patients with Rheumatoid Arthritis
Description	Ex vivo T cell treatment Immunomagnetic negative isolation was used to obtain untouched human T cells from previously frozen PBMC samples of treatment-naïve (n=6) and MTX-IR (n=3) RA patients (Table S1). The isolated T cells were then cultured and stimulated in a 96-well round-bottom plates for suspension cells in a concentration of 1.5×105 cells per well in full HPLM and CD3/CD28 Dynabeads as described above in the presence of TH9619 (1uM) or MTX (1uM) or DMSO (vehicle control) for 24 hours. After incubation, cells were washed twice with DPBS, pelleted, snap-frozen, and stored for subsequent lysis and processing.
HostingRepository	PRIDE
AnnounceDate	2026-04-02
AnnouncementXML	Submission_2026-04-02_09:17:10.560.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martina Samiotaki
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-14 09:22:32	ID requested
⏵ 1	2026-04-02 09:17:11	announced

Dataset with its publication pending

submitter keyword: proteomics, methotrexate,selective MTHFD1/2 inhibitor, T cells, rheumatoid arthritis

Thomas Helleday
contact affiliation	Department of Oncology-Pathology, Karolinska Institutet Stockholm, Sweden
contact email	thomas.helleday@scilifelab.se
lab head
Martina Samiotaki
contact affiliation	Protein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact email	samiotaki@fleming.gr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD061849

PRIDE project URI

• PRIDE
  1. PXD061849
     1. Label: PRIDE project
     2. Name: Selective Inhibition of MTHFD1/2 in T Cells from Patients with Rheumatoid Arthritis