Large-scale plasma proteomics studies can advance the human proteome atlas mapping, facilitating precision medicine, but are constrained by the low efficiency of sample pretreatment. Here, we reported a fully automated plasma proteomic platform based on the All-in-Tip, enabling the whole sample pretreatment to be performed at room temperature within 1 hour. The All-in-Tip used functional macroporous nanoparticles (NPs) to form protein corona in 5 minutes to narrow the abundance range of plasma proteins and the spatial confinement of macroporous NPs to offer efficient digestion in 5 minutes. The All-in-Tip workflow is highly coupled with automation to achieve high reproducibility (correlation > 0.95, median coefficient of variation < 30%) proteomic profiling. Our strategy was validated in the early-stage lung adenocarcinoma cohort, annotating over 5100 protein groups, screening 353 differentially expressed proteins, and distinguishing early-stage lung adenocarcinoma from pulmonary benign diseases with an AUC of 0.89. The All-in-Tip workflow offers high-throughput, robust and deep profiling, which meets the requirements of large-scale clinical cohorts and thus promises to advance the elucidation of disease mechanisms and new biomarker discovery.