PXD061171

PXD061171 is an original dataset announced via ProteomeXchange.

Title	Redox control of antibiotic biosynthesis in Streptomyces formicae
Description	Streptomyces bacteria make diverse specialised metabolites that form the basis of ~55% of clinically used antibiotics. Despite this, only 3% of their encoded specialised metabolites have been matched to molecules and understanding how their biosynthesis is controlled is essential to fully exploit their potential. Here we use Streptomyces formicae and the formicamycin biosynthetic pathway as a model to understand the complex regulation of specialised metabolism. We analysed all three pathway-specific regulators and found that biosynthesis is subject to negative feedback and redox control via two MarR-family proteins while activation of the pathway is dependent on a cytoplasmic two-component system. Like many Streptomyces antibiotics, formicamycins are only produced in solid culture and biosynthesis is switched off in aerated liquid cultures. Here, we demonstrate that a redox-sensitive repressor named ForJ senses oxygen via a single cysteine residue that is required to repress formicamycin biosynthesis in liquid cultures.
HostingRepository	PRIDE
AnnounceDate	2025-08-05
AnnouncementXML	Submission_2025-08-05_04:00:18.778.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061171
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gerhard Saalbach
SpeciesList	scientific name: Streptomyces formicae; NCBI TaxID: 1616117;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-25 05:11:13	ID requested
⏵ 1	2025-08-05 04:00:19	announced

10.6019/PXD061171;

submitter keyword: proteomics, antibiotic biosynthesis, redox,Formicamycins, regulation

Matt Hutchings
contact affiliation	John Innes Centre Dept. Molecular Microbiology Head of Department Norwich UK
contact email	matt.hutchings@jic.ac.uk
lab head
Gerhard Saalbach
contact affiliation	Biological Chemistry
contact email	gerhard.saalbach@jic.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061171
     1. Label: PRIDE project
     2. Name: Redox control of antibiotic biosynthesis in Streptomyces formicae