This study employs DARTS (Drug Affinity Responsive Target Stability) to investigate capsazepine's targets. The technique detects drug-induced proteolytic resistance in target proteins, coupled with mass spectrometry to capture drug-protein complexes. Experiments focus on identifying novel targets beyond TRPV1 (e.g., G protein-coupled receptors) to elucidate its analgesic mechanisms and polypharmacological effects. Key findings may advance pharmacological optimization of analgesics and expand therapeutic applications.