The effect of CBD was investigated in a preclinical model of PCOS generated by chronic exposure of female mice to DHT from weaning onwards. To exacerbate the metabolic complications in this model, PWA mice were fed a high-fat diet (HFD) for 18 weeks before initiation of the pharmacological treatments. This model phenocopies a subtype of women with this condition who suffer metabolic comorbidities. PWA exacerbated the metabolic disturbances induced by the high-fat diet (HFD), as HFD-fed, non-androgenized female mice exhibited a smaller increase in body weight (BW) compared to androgenized females on the same diet before treatment initiation (Fig. 1A). Daily treatment with CBD for 28 days caused a clear reduction of BW through the treatment period. This datset contains the results of a proteomic characterization of the livers from control mice, PWA mice and CBD treated mice.