Nephrotic syndrome is a severe disease that significantly impairs kidney function, potentially leading to renal failure and a high mortality rate. Recent studies have shown that mutations in the kidney-specific protein Podocin are closely associated with the onset of this disease, with the R138Q-Podocin mutation being particularly implicated in its pathogenesis. This study aims to compare the interactome of R138Q-Podocin with that of wild-type (WT) Podocin to elucidate its pathogenic mechanisms and explore potential therapeutic strategies.