PXD060764

PXD060764 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-based profiling of HDF, HDF Vim -/-, and their EVs, and the impact of these EVs on MCF7 and MCF10A proteomes
Description	Vimentin is a well-established intracellular regulator of epithelial-mesenchymal transition (EMT). Recent studies have highlighted the role of extracellular vimentin from fibroblast-derived extracellular vesicles (EVs) in promoting wound healing and other functions. Building on these findings, we investigated the protein cargo of wild-type (WT) and vimentin-knockout (Vim -/-) human dermal fibroblasts (HDFs) and their EVs, aiming to determine whether extracellular vimentin influences EMT. Additionally, we assessed the impact of EVs from WT and Vim -/- HDFs on the proteomes of MCF7 and MCF10A cells
HostingRepository	PRIDE
AnnounceDate	2025-07-10
AnnouncementXML	Submission_2025-07-10_02:47:38.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Santos Silva
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-13 04:02:44	ID requested
⏵ 1	2025-07-10 02:47:38	announced

10.1016/J.MCPRO.2025.101028;

submitter keyword: Extracellular vesicles, Extracellular vimentin, LC-MS/MS

John Eriksson
contact affiliation	Åbo Akademi University, Faculty of Science and Engineering, Finland
contact email	john.eriksson@abo.fi
lab head
Michael Santos Silva
contact affiliation	Åbo Akademi University
contact email	msantoss@abo.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD060764

PRIDE project URI

• PRIDE
  1. PXD060764
     1. Label: PRIDE project
     2. Name: Mass spectrometry-based profiling of HDF, HDF Vim -/-, and their EVs, and the impact of these EVs on MCF7 and MCF10A proteomes